Analysis of phytochemical composition and biological activities of Helichrysum pallasii (Sprengel) ledeb leaves

This study aimed at the investigation of in vitro biological activities (antioxidant and enzyme inhibition) phytochemical composition various extracts (ethanol maceration, aqueous infusion, decoction) obtained from leaf organs Helichrysum pallasii, which has been traditionally used as herbal tea medicine Eastern Anatolia. Experimental analysis showed that ethanol-based extract had higher total phenolic content (TPC: 103 mg GAE/gE) superior antioxidant potentials (FRAP: 2205 μmol Fe2+/gE; ORAC: 2540 Trolox Eq./gE; DPPH: IC50=0.58 mg/ml; CUPRAC: IC50=0.37 Phosphomolybdenum: IC50=1.34 mg/ml ve metal chelation: IC50=1.42 mg/ml) inhibition (Acetylcholinesterase: IC50=1.49 Butyrylcholinesterase: IC50=1.98 Tyrosinase: IC50=0.68 Alpha-amylase: IC50=2.09 Alpha-glucosidase: IC50=0.51 Pancreatic lipase: IC50=42.5 μg/ml) contained amounts (chlorogenic acid isomers rutin) fatty (palmitic, linoleic, linolenic acids) compounds than traditional preparations (infusion decoction). The current study's findings indicate leaves pallasii are a source phytochemicals with strong inhibitory properties, implying it could be candidate for biotherapeutic agent research development.